Evaluation of transfection methods for transient gene expression in Chinese hamster ovary cells

نویسندگان

  • Si Nga Sou
  • Karen M. Polizzi
  • Cleo Kontoravdi
چکیده

Three transfection reagents, Lipofectamine 2000, TransIT-PRO and linear 25 kDa polyethylenimine were evaluated for transient expression of enhanced green fluorescent protein in Chinese hamster ovary cells. TransIT-PRO was found to be more efficient under the examined conditions, but comes at an increased cost compared to the widely used PEI.

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تاریخ انتشار 2013